Molecular Microbiology and Genomics Consultants

Selected Publications

• Comparative genomics of Bifidobacterium, Lactobacillus and related probiotic genera.
  Lukjancenko, O. Ussery, D.W. and Wassenaar, T.M.
  (2011) Microb. Ecol. PDF - complete
• Following an imaginary Campylobacter population from farm to fork and beyond: a bacterial perspective.
  Wassenaar, T.M.
  (2011) J Appl Microbiol.. 53:253-263. PDF - first page
• The thousandth genome - a cautionary tale.
  Lagesen, K., Ussery, D.W., and Wassenaar, T.M.
  (2010). Microbiology 156:603-608. PDF - first page
• Comparison of 61 sequenced Escherichia coli genomes.
  Lukjancenko, O., Wassenaar, T.M. and Ussery, D.W.
  (2010). Microb Ecol. 60:708-720. PDF - first page
• Genomic characterization of Campylobacter jejuni strain M1.
  Friis, C., Wassenaar, T.M., Javed, M.A., Snipen, L., Lagesen, K., Newell, D.G., Manning, G. and Ussery, D.W.
  (2010). PLoS One 5(8): e12253. PDF - complete
• Comparison of Campylobacter fla-SVR genotypes isolated from humans and poultry in three European regions.
  Wassenaar, T.M., Fernández-Astorga, A., Alonso, R., Marteinsson, V.T., Magnússon, S.H., Kristoffersen,
  A.B. and Hofshagen, M.(2009). Lett. Appl. Microbiol. 49:388-395 PDF - first page
• On the origins of a Vibrio species.
  Vesth, T., Wassenaar, T.M., Hallin, P., Snipen, L., Lagesen, K. and Ussery, D.W.
  (2009). Microb. Ecol. 59:1-13. PDF - first page
• The genus Burkholderia: analysis of 56 genomic sequences.
  Ussery, D.W., Kiil, K., Lagesen, K., Sicheritz-Pontén, K., Bohlin, J. and Wassenaar, T.M.
  (2009). Genome Dyn. 6:140-157. PDF - first page
• Genome comparison of bacterial pathogens.
  Wassenaar, T.M., Bohlin J., Binnewies T.T. and Ussery D.W.
  (2009). Genome Dyn. 6:1-20. PDF - first page
• Antimicrobal resistance in zoonotic bacteria: lessons learned from host-specific pathogens.
  Wassenaar TM and Silley P.
  (2008). Animal Health Res. Rev. 92:177-186. PDF - first page
• Safety aspects of bacterial food supplements
  Wassenaar TM and Klein G.
  (2008). J. Food Prot. 71:1734-1741. PDF - first page
• Putative identification of an amphipatic, alpha-helical sequence inhemolysin of E. coli (HlyA) involved in transmembrane pore formation. Valeva A, Siegel I, Wylenzek M, Wassenaar TM, Weis S, Heinz N, Schmitt R, Fischer C, Reinarzt R, Bhakdi S and Walev I.
  (2008). Biol. Chem. 389: 1201-1207. PDF - first page
• The identification of hyperinvasive Campylobacter jejuni strains in poultry and human clinical isolates. Fearnley C, Manning G, Bagnall M, Javed MA, Wassenaar TM and Newell DG,
  (2008). J. Med. Microbiol. 57:570-580.
• Genetic instability is associated with changes in the colonization potential of Campylobacter jejuni in the avian intestine. Ridley AM, Toszeghy MJ, Cawthraw, SA, Wassenaar TM, and Newell DG.
  (2008). J. Appl. Microbiol. 105:95-104. PDF - first page
• Development of a strain-specific molecular method for quantitating individual campylobacter strains in mixed poopulations. Elvers KT, Helps CR, Wassenaar TM, Allen VM and Newell DG.
  (2008). Appl. Environm. Microbiol. 74:2321-2331. PDF - first page
• The importance of virulence prediction and gene networks in microbial risk assessment. Wassenaar, T.M., Gamieldien, J., Shatkin, J., Luber, P., Moyer, N., Carpenter, T. and Ussery, D. W. (2007) Human and Ecological Risk Assessment. 2: 254-268.
• Characterization of probiotic Escherichia coli isolates with a pan-genome microarray. Willenbrock H, Hallin PF, Wassenaar TM, and Ussery DU.
  (2007). Genome Biol, 8:R267. PDF - first page
• Pro-inflammatory feedback activation cycle evoked by attack of Vibrio cholerae cytolysin on human neutrophil granulocytes. Valeva A, Walev I, Weis S, Boukhallouk F, Wassenaar TM, and Bhakdi S.
  (2007). Med. Microbiol. Immunol. 197:285-293.
• Re-analysis of the risks attributed to ciprofloxacin-resistant Campylobacter jejuni infections. Wassenaar, TM, Kist M, and de Jong A.
  (2007). Int. J. Antimicrob. Agents 30:195-201. PDF - first page
• Genotyping of Human and Porcine Yersinia enterocolitica, Yersinia intermedia and Yersinia bercovieri Strains from Switzerland by Amplified Fragment Length analysis Polymorphism (AFLP). Boghenbor KK, On SLW, Kokotovic B, Frey J, Baumgartner A, Wassenaar TM, Wittwer M, Bissig-Choisat B.
  (2006). Appl. Environm. Microbiol. 72:4061-4066. PDF - first page
• Ten years of bacterial genomes sequencing: comparative genomics based discoveries. Binnewies TT, Motro Y, Hallin PF, Lund O, Dunn D, Hampson D, Bellgard M, Wassenaar TM, and Ussery DW.
  (2006) Funct. Integr. Genomics 6:165-185. PDF - first page
• Phenon cluster analysis as a method to investigate epidemiological relatedness between sources of Campylobacter jejuni. Wieland B, Wittwer M, Regula G, Wassenaar TM, Burnens AP, Keller J, and StŠrk KDC.
  (2006) J. Appl. Microbiol. 100:316-324. PDF - first page
• Evidence of host-virus co-evolution in tetranucleotide usage patterns of bacteriophages and eukaryotic viruses. Pride D, Wassenaar TM, Ghose C, and Blaser MJ.
  (2006) BMC Genomics 7:8. PDF - first page
• Detection of Mycobacterium avium subspecies paratuberculosis in Swiss dairy and mother cattle by real-time PCR, culture and serology. Bšgli-Stuber K, Kohler C, Seitert G, Glanemann B, Antognoli M., Salman MD, Wittenbrink MM, Wittwer M, Wassenaar T, Jemmi T, and Bissig-Choisat B.
  (2005). J. Appl. Microbiol. 99:587-597.
• Role of dprA in transformation in Campylobacter jejuni. Takata T, Ando T, Israel D, Wassenaar TM and Blaser MJ.
  (2005) FEMS Microbiol. Lett. 252:161-168. PDF - first page
• Genome update: sigma factors in 240 bacterial genomes. Kill K, Binnewies TT, Sicheritz-Ponten T, Willenbrock H, Hallin PF, Wassenaar TM and Ussery DW.
  (2005) Microbiology. 151:3147-3150. PDF - first page
• Phage therapy reduces Campylobacter jejuni colonization in broilers. Wagenaar JA, Van Bergen MAP, Mueller MA, Wassenaar TM and Carlton RM.
  (2005) Vet. Microbiol. 109:275-283. PDF - first page
• Campylobacter spp. in dogs and cats in Switzerland: risk factor analyses and AFLP characterization. Wieland B, Regula G, Danuser J, Wittwer M, Burnens AP, Wassenaar TM and Stärk KDC.
  (2005) J. Vet. Med. Series B 52:183-189.
• Binding of E. coli hemolysin and activation of the target cells is not receptor-dependent. Valeva A, Walev I, Kemmer H, Weis S, Siegel I, Wassenaar TM, Chavakis T and Bhakdi S. 
  (2005) J. Biol. Chem. 280:36657-36663. PDF - first page
• Genome update: protein secretion systems in 225 bacterial genomes. Binnewies TT, Bendsen JD, Hallin PF, Nielsen N, Wassenaar TM, Pedersen MB, Klemm P and Ussery DW.
  (2005)   Microbiology UK. 151:1013-1016. PDF - first page
• Identification of the membrane penetrating domain of Vibrio cholerae cytolysin as a beta-barrel structure. Valeva A, Walev I, Boukhallouk F, Wassenaar TM, Heinz N,, Hedderich J, Lautwein S, Möcking M, Weis S, Zitzer A and Bhakdi S.
  (2005) Mol. Microbiol. 57:124-131. PDF - first page
• Use of antimicrobial agents in veterinary medicine and implications to human health. Wassenaar TM.
  (2005) Critical Reviews in Microbiology 31:155-169. PDF - first page
• Cytolethal Distending Toxin (CDT) negative Campylobacter jejuni Strains and anti-CDT antibodies induced during human infection. AbuOun M, Manning G, Cawthraw SA, Ridley A, Ahmed IH, Wassenaar TM and Newell DG.
  (2005) Infect. Immun. 73:3053-3062. PDF - first page
• Genetic diversity and antibiotic resistance patterns in a Campylobacter population isolated from poultry farms in Switzerland. Wittwer M, Keller J, Wassenaar TM, Stephan R, Howald D, Regula G, and Bissig-Choisat B.
  (2005). Appl. Environm. Microbiol. 71:2840-2847. PDF - first page
• Detection of Mycobacterium avium subspecies paratuberculosis in Swiss dairy and mother cattle by real-time PCR, culture and serology. Bögli-Stuber K, Seitert G, Kohler C, Glanemann B, Antognoli MC, Salman MD, Wittenbrink MM Wittwer M, Wassenaar T, and Bissig-Choisat B.
  (2005) J. Appl. Microbiol. 99:587-597.
• Genome Update: tRNA's in sequenced microbial genomes. Ussery DW, Hallin PF, Lagesen K and Wassenaar TM.
  (2004). Microbiology UK. 150:1113-1115. PDF - first page
• A cellular protease activates Vibrio cholerae pro-cytolysin. Valeva A, Walev I, Weis S, Boukhallouk F, Wassenaar TM, Bhakdi S and Zitzer A.
  (2004) J. Biol. Chem. 279:25143-25148. PDF - first page
• Risk Assessment prediction from genome sequences, promises and dreams. Wassenaar TM.
  (2004) J. Food Protection 67:2053-2057. PDF - first page
• The TGA stop codon and the phylogeny of the selenocysteine pathway. Wassenaar TM and Meinersmann RJ. (2003) Genome Letters. 2:127-138.
• Patterns in the intergenic regions of Campylobacter jejuni. Meinersmann RJ and Wassenaar TM.
  (2003) Genome Letters 2:28-33.
• Molecular typing of pathogens. Wassenaar, T.M.
  (2003) Berl. Muench. Tieraertzl. Wbl. 116:447-453. PDF - first page
• Structure and genotypic plasticity of the Campylobacter fetus sap locus. Tu ZC, Wassenaar TM, Thompson SA and Blaser MJ
  (2003) Mol. Microbiol. 48:685-698. PDF - first page
• PCR detection of virulence genes in Yersinia enterocolitica and Yersinia pseudotuberculosis and investigation of virulence gene distribution. Thoerner P, Bin Kingombe CI, Bissig-Choisat B, Bögli-Stuber K, Wassenaar TM, Frey J and Jemmi T
  (2003) Appl. Environm. Microbiol. 69:1810-1816. PDF - first page
• Evolutionary implications of microbial genome tetranucleotide frequency biases. Pride DT, Meinersmann RJ, Wassenaar TM and Blaser MJ
  (2003) Genome Research 13:145-158. PDF - first page
• Evolutionary history of hrgA, which replaces the restriction gene hpyIIIR in the hpyIII locus of H. pylori. Ando T, Aras RA, Kusugami K,  Blaser MJ and Wassenaar TM
  (2003) J. Bacteriol. 185:295-301. PDF - first page
• Homonucleotide stretches in chromosomal DNA of Campylobacter jejuni display high frequency polymorphism as detected by direct PCR analysis. Wassenaar TM, Wagenaar JA, Rigter A, Fearnley C, Newell DG and Duim B
  (2002)   FEMS Microbiol Lett. 212:77-85. PDF - first page
• Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR-Based Restriction Fragment Length Polymorphism Analysis. Feng S, Yuen YC, Wassenaar TM, Woods WH, Coloe PJ  and Fry BN
  (2002) J. Clin. Microbiol. 40:1791-1797. PDF - first page
• A Helicobacter pylori restriction endonuclease replacing gene, hrgA, associated with gastric cancer in Asian strains. Ando T, Wassenaar TM, Peek RM, Aras RA, Tschumi AI, van Doorn L-J, Kusugami K, and Blaser MJ
  (2002) Cancer Research 62:2385-2389. PDF - first page
• Contagion on the internet. Wassenaar TM and Blaser MJ
  (2002) Emerging Infect. Dis. 8: 335-336. PDF - first page
• Identification of genetic differences between two Campylobacter jejuni strains with different colonisation potentials. Ahmed I, Manning G, Wassenaar T, Crawthraw S and Newell D
  (2002) Microbiology UK 148:1203-1212. PDF - first page
• Bacterial virulence, where to draw the line? FEMS Microbiol. Wassenaar TM and Gaastra W.
  (2001) Letters 201:1-7. PDF - first page
• Evidence for a genetically stable clone of Campylobacter jejuni. Manning G, Duim B, Wassenaar TM, Wagenaar JA, Ridley A and Newell DG.
  (2001) Appl. Environm. Microbiol. 67:1185-1189.
• Genetic characterisation of Campylobacter jejuni O:41 in relation to Guillain Barré Syndrome. Wassenaar TM, Lastovica A, Duim B, Wagenaar J and Fry B
  (2000) J. Clin. Microbiol. 38: 874-876. PDF - first page
• Genotyping of Campylobacter spp. Wassenaar TM and Newell DG.
  (2000) Appl. Environm. Microbiol. 66: 1-9. PDF - first page
• Pathophysiology of Campylobacter infections of humans. Wassenaar TM and Blaser MJ
  (1999) Microbes Infect. 1: 1023-1033. PDF - first page
• High-resolution genotyping of Campylobacter strains isolated from poultry and humans with AFLP fingerprinting. Duim B, Wassenaar TM, Rigter A and Wagenaar J
  (1999) Appl. Environm. Microbiol. 65: 369-2375. PDF - first page
• Evidence of genomic instability in Campylobacter jejuni isolated from poultry meat. Wassenaar TM, Geilhausen B and Newell DG
  (1998) Appl. Environm. Microbiol. 64: 1816-1821. PDF - first page
• Differential up uptake and killing potential of Campylobacter jejuni by human peripheral monocytes/macrophages. Wassenaar TM, Engelskirchen M, Park S and Lastovica A
  (1997) Med. Microbiol. Immunol. 186: 139-144.
• Mode of primary binding to target membranes and pore formation induced by Vibrio cholerae cytolysin (hemolysin). Zitzer A, Palmer M, Weller U, Wassenaar T, Biermann C, Tranum-jensen J and Bhakdi S
  (1997) Eur.J. Biochem. 247: 209-216.
• Potent membrane-permeabilizing and cytocidal action of Vibrio cholerae cytolysin (hemolysin) on human intestinal cells Infect. Zitzer A, Wassenaar TM, Walev I and Bhakdi S
  (1997) Immun. 65: 1293-1298. PDF - first page
• Toxin production by Campylobacter spp. Wassenaar TM
  (1997) Rev. Clin. Microbiol. 10: 466-476. PDF - first page
• Increased colonization potential of Campylobacter jejuni strain 81116 after passage through chickens and its implication on the rate of transmission within flocks. Cawthraw SA, Wassenaar TM, Ayling R and Newell DG
  (1996) Epidemiol. Infect. 117: 213-215.
• Variation of the flagellin gene locus of Campylobacter jejuni by recombination and horizontal gene transfer. Wassenaar TM, Fry BN and Van der Zeijst BAM
  (1995) Microbiology 141: 95-101.
• Pathogenitätsfaktoren von enteritischen Salmonellen. Loos M and Wassenaar TM
  (1994) Immunität und Infektion, 22: 14-19.
• Differential flagellin expression in a flaAflaB+ mutant of Campylobacter jejuni. Wassenaar TM, Bleumink-Pluijm NMC, Newell DG, Nuijten PJM and Van der Zeijst BAM
  (1994) Infect. Immun. 62: 3901-3906.
• Isotype, specificity, and kinetics of systemic and mucosal antibodies to Campylobacter jejuni antigens, including flagellin, during experimental oral infections of chickens. Cawthraw SA, Ayling R, Nuijten P, Wassenaar T and Newell DG
  (1994). Avian Dis. 38: 341-349.
• Colonization of chicks by motility mutants of Campylobacter jejuni demonstrates the importance of flagellin A expression. Wassenaar TM, Van der Zeijst BAM, Ayling R and Newell DG
  (1993) J.Gen. Microbiol. 139: 1171-1175.
• Genetic manipulation of Campylobacter: evaluation of natural transformation and electro-transformation. Wassenaar TM, Fry BN and Van der Zeijst BAM
  (1993) Gene 132, 131-135
• Inactivation of Campylobacter jejuni flagellin genes by homologous recombination demonstrates that flaA but not flaB is required for invasion. Wassenaar TM, Bleumink-Pluym NMC and Van der Zeijst BAM
  (1991)EMBO J 10: 2055-2061.
• Variations in Campylobacter jejuni flagellin, and flagellin genes, during in vivo and in vitro passage. King V, Wassenaar T, Van der Zeijst BAM and Newell DG
  (1991) Microb. Ecol. Health Dis. 4: 135-140.
• The extended promoter of the gene encoding the ribosomal protein S33 in yeast consists of multiple binding elements. Herruer MH, Mager WH, Doorenbosch TM, Wessels PLM, Wassenaar TM and Planta RJ
  (1989) Nucl. Acids Res. 17: 7427-7439.
• Cytidylate cyclase activity: identification of cytidine 3',5'-cyclic monophosphate and four novel cytidine cyclic phosphates as biosynthetic products from cytidine triphosphate. Newton, R.P., Hakeem, N.A., Salvage, B.J., Wassenaar, G., and Kingston, E.E.
  (1988) Rapid Commun. Mass Spectrom. 2:118-126.
• Transcriptional control of yeast ribosomal protein synthesis during carbon-source upshift. Herruer MH, Mager WH, Woudt LP, Nieuwint RTM, Wassenaar GM, Groenevelt P and Planta RJ
  (1987) Nucl. Acids Res. 15: 10133-10144.
• Structural and putative regulatory sequences of the gene encoding ribosomal protein L25 in Candida utilis. Woudt LP, Mager WH, Beek JG, Wassenaar GM and Planta RJ
  (1987) Curr. Genet. 12: 193-198.
• Analysis of upstream activation sites of yeast ribosomal protein genes. Woudt LP, Mager WH, Nieuwint RTM, Wassenaar GM, Van der Kuyl AC, Murre JM, Hoekman MFM, Brockhoff PGM and Planta RJ
  (1987) Nucl. Acids Res. 15: 6037-6048.
• Specific binding of TUF factor to upstream activation sites of yeast ribosomal protein genes. Vignais ML, Woudt LP, Wassenaar GM, Mager WH, Sentenac A, Planta RJ
  (1987) EMBO J. 6: 1451-1457.
• Mitochondrially encoded resistance to Paromomycine in Saccharomyces cerevisiae: reinvestigation of a controversy. Tabak HF, Van der Laan JC, Landegent JE, Evers RF and Wassenaar GM
  (1982) Plasmids 8: 261-275